Trypsin 0.05 % / EDTA 0.02 % in DPBS
Trypsin (0.05 %) / EDTA (0.02 %) in DPBS for enzymatic cell dissociation. · Cell dissociation solution · RUO (P10-023100) · GMP (CT-023100)
Trypsin (0.05 %) / EDTA (0.02 %) in DPBS is a ready-to-use dissociation solution for the gentle enzymatic detachment of adherent cells from culture surfaces. Trypsin cleaves the peptide bonds anchoring the cells, while the chelating agent EDTA binds divalent cations and thereby enhances dissociation. After detachment the trypsin should be neutralised, for example with serum or a trypsin inhibitor.
PAN-Biotech quality. Trypsin 0.05 % / EDTA 0.02 % in DPBS is manufactured by PAN-Biotech / PAN-Celltech and released against a defined quality-control panel; the matched GMP grade is available as CT-023100.
Why
- Enzymatic dissociation – detaches adherent cells.
- 0.05 % / 0.02 % – defined trypsin/EDTA concentration.
- In DPBS – Ca/Mg-free base.
- GMP on request – for regulated processing.
Specification
| Composition | 0.05 % trypsin / 0.02 % EDTA in DPBS |
| Application | Cell detachment / passaging |
| Form | Solution |
| Grade | RUO & GMP |
| Storage | -20 °C |
Ordering
In the process – by application (upstream & downstream)
Trypsin 0.05 % / EDTA 0.02 % in DPBS is used at the following process steps across the biologics platforms it supports:
Cell & gene therapy
- Dissociation – Enzymatic dissociation.
Vaccines
- Cell dissociation – Enzymatic dissociation.
Frequently asked questions
What is this used for?
Enzymatic detachment and passaging of adherent cells.
What is the concentration?
0.05 % trypsin with 0.02 % EDTA in DPBS.
In which grade is Trypsin 0.05 % / EDTA 0.02 % in DPBS available?
It is available as a RUO product (P10-023100); a matching GMP grade (CT-023100) is also available.
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Related: Cell & Gene Therapy · Cell-Specific Media