Tris Buffer 1 M, pH 8.4
1 M Tris-HCl buffer concentrate at pH 8.4 for equilibration and neutralisation in downstream processing. · GMP catalogue product · CT-05500
Tris Buffer 1 M, pH 8.4 is a defined buffer concentrate for downstream processing. It is diluted to working strength for column equilibration and used for neutralisation / pH re-adjustment, for example after a low-pH viral-inactivation hold. As a concentrated, GMP-grade base it is used across monoclonal antibodies, vaccines, mRNA and cell & gene therapy.
One GMP quality. Tris Buffer 1 M, pH 8.4 is a GMP-grade catalogue product (CT-05500), manufactured by PAN-Celltech and released against a defined quality-control panel on every lot.
Why
- Concentrated 1 M base – Dilute to the required working strength.
- Reliable neutralisation – Defined pH 8.4 ± 0.2 for post-inactivation adjustment.
- GMP grade – Catalogue GMP quality, released per lot.
- Multi-application – One base across antibody, vaccine, mRNA and C&G downstream.
Specification
| Composition | 1 M Tris-HCl |
| pH | 8.4 ± 0.2 |
| Form | Buffer concentrate |
| Storage | 15-25 °C (room temperature) |
| Grade | GMP |
Ordering
| Grade | Article No. | Pack size |
|---|---|---|
| GMP | CT-05500 | 500 ml |
Other pack sizes are available on request.
Beyond compendial – on request. Every PAN downstream buffer is also available in a beyond-compendial version produced with WFI Beyond – Water for Injection engineered beyond the Ph. Eur./USP monograph – for the most demanding endotoxin and purity requirements. Available on request.
In the process – by application (upstream & downstream)
Tris Buffer 1 M, pH 8.4 is used at the following process steps across the biologics platforms it supports:
Monoclonal antibodies
- Protein A capture – equilibration buffer base.
- Viral inactivation – neutralisation / pH re-adjustment after the low-pH hold.
- Ion exchange – equilibration and pH adjustment.
Vaccines
- Chromatography – equilibration buffer base.
- Post-treatment – neutralisation and pH adjustment.
mRNA
- Handling / storage – TE-type buffering (Tris base).
- Buffer exchange / formulation – buffering to target pH.
- Reactions – neutralisation / reaction stop.
Cell & gene therapy
- Chromatography – equilibration and neutralisation.
- Lysis / handling – TE-buffer component.
- Plasmid DNA – process buffering.
Frequently asked questions
What is Tris Buffer 1 M, pH 8.4 used for?
It is a concentrate for column equilibration and for neutralisation / pH re-adjustment after low-pH steps.
What is the pH?
The concentrate is supplied at a fixed pH of 8.4 ± 0.2.
Is it multi-application?
Yes. It is used across monoclonal antibodies, vaccines, mRNA and cell & gene therapy downstream.
One partner across every application. The same GMP downstream buffers support monoclonal antibodies, vaccines, mRNA and cell & gene therapy – explore the full GMP-grade catalogue or use the Smart Product Search.
Related: WFI Quality Water (CT-991500) · DPBS w/o Ca & Mg (CT-36500) · GMP-Grade Products