Upstream & Downstream

MEM Eagle w: EBSS, w: L-Glutamine, w: 2.2 g/L NaHCO₃

Classic cell culture medium · RUO (P04-08500) · GMP (CT-08500)

GMP · CT-08500
RUO · P04-08500
Upstream · USP

Minimum Essential Medium (MEM), developed by Harry Eagle, is a widely used synthetic basal medium and an advancement of Basal Medium Eagle (BME). This formulation contains Earle’s Balanced Salt Solution (EBSS), L-glutamine, glucose and 2.2 g/L sodium bicarbonate, with phenol red and without HEPES. It supports a broad range of mammalian cells and is readily supplemented with amino acids or small amounts of serum.

One GMP quality. MEM Eagle w: EBSS, w: L-Glutamine, w: 2.2 g/L NaHCO₃ is a GMP-grade catalogue product (CT-08500), manufactured or assured by PAN-Celltech and released against a defined quality-control panel on every lot.

Why

  • Widely used synthetic medium – one of the most-used base media for mammalian cells.
  • EBSS-buffered – with L-glutamine and 2.2 g/L sodium bicarbonate.
  • Adherent-cell friendly – suitable for virus propagation on cell substrates.

Specification

Base MEM Eagle (Minimum Essential Medium) with Earle's Balanced Salt Solution (EBSS)
Key features With L-glutamine; 2.2 g/L NaHCO₃
Form Liquid medium
Grade RUO & GMP
Storage 2-8 °C, protected from light

Ordering

Grade Art. No. Pack size To Shop
RUO P04-08500 500 ml
GMP CT-08500 500 ml

Other pack sizes on request.

In the process – by application (upstream & downstream)

MEM Eagle w: EBSS, w: L-Glutamine, w: 2.2 g/L NaHCO₃ is used at the following process steps across the biologics platforms it supports:

Cell & gene therapy

  • Vector Production / Transfection – Adherent producer-cell substrate for viral vector production.

Vaccines

  • Cell-line-specific viral culture media

Frequently asked questions

Which processes is this product used in?

MEM Eagle w: EBSS, w: L-Glutamine, w: 2.2 g/L NaHCO₃ is used across the PAN Monoclonal antibody and Cell & gene therapy processes. See the section above for the specific steps in each.

In which grade is MEM Eagle available?

It is available as a RUO product (P04-08500); a matching GMP grade (CT-08500) is also available.

One partner across every application. Explore the full GMP-grade catalogue or use the Smart Product Search.