M199 w: EBSS, w: L-Glutamine, w: 2.2 g/L NaHCO₃
Classic cell culture medium · RUO (P04-07050) · GMP (CT-07050)
Medium 199 (M199) was originally developed to assay the nutrient demand of embryonic chicken fibroblasts and works well with cells from many animal species, including in virology and vaccine production. This formulation contains Earle’s Balanced Salt Solution (EBSS), L-glutamine, glucose and 2.2 g/L sodium bicarbonate, with phenol red and without HEPES. For long-term culture, serum should be added.
One GMP quality. M199 w: EBSS, w: L-Glutamine, w: 2.2 g/L NaHCO₃ is a GMP-grade catalogue product (CT-07050), manufactured or assured by PAN-Celltech and released against a defined quality-control panel on every lot.
Why
- Classic versatile medium – suitable for many mammalian and primary cells.
- EBSS-buffered – with L-glutamine and 2.2 g/L sodium bicarbonate.
- Established for virology – a long-standing medium for vaccine production.
Specification
| Base | M199 (Medium 199) with Earle's Balanced Salt Solution (EBSS) |
| Key features | With L-glutamine; 2.2 g/L NaHCO₃ |
| Form | Liquid medium |
| Grade | RUO & GMP |
| Storage | 2-8 °C, protected from light |
Ordering
In the process – by application (upstream & downstream)
M199 w: EBSS, w: L-Glutamine, w: 2.2 g/L NaHCO₃ is used at the following process steps across the biologics platforms it supports:
Cell & gene therapy
- Vector Production / Transfection – Adherent producer-cell substrate for viral vector production.
Vaccines
- Cell-line-specific viral culture media
Frequently asked questions
Which processes is this product used in?
M199 w: EBSS, w: L-Glutamine, w: 2.2 g/L NaHCO₃ is used across the PAN Monoclonal antibody and Cell & gene therapy processes. See the section above for the specific steps in each.
In which grade is M199 available?
It is available as a RUO product (P04-07050); a matching GMP grade (CT-07050) is also available.
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