EBSS, w/o Ca and Mg, w: NaHCO₃
Earle Balanced Salt Solution without calcium and magnesium, with sodium bicarbonate. · Balanced salt solution · RUO (P04-31500)
Earle’s Balanced Salt Solution (EBSS) is an isotonic balanced salt solution of inorganic salts and glucose, buffered with sodium bicarbonate. It maintains the physiological pH and osmotic balance of cells and is used to wash and rinse cells and tissues. It also serves as a basis for more complex culture media and reagents.
PAN-Biotech quality. EBSS, w/o Ca and Mg, w: NaHCO₃ is manufactured by PAN-Biotech / PAN-Celltech and released against a defined quality-control panel; a GMP grade is available on request where applicable.
Why
- Without Ca and Mg – for dissociation-support handling.
- With NaHCO₃ – bicarbonate-buffered.
- Isotonic wash – cell washing and handling.
- GMP on request – for regulated processing.
Specification
| Composition | Earle balanced salts, w/o Ca and Mg, w: NaHCO₃ |
| Application | Washing / handling |
| Form | Solution |
| Grade | RUO |
| Storage | 2-8 °C |
Ordering
| Grade | Art. No. | Pack size |
|---|---|---|
| RUO | P04-31500 | 500 ml |
Other pack sizes are available on request.
In the process – by application (upstream & downstream)
EBSS, w/o Ca and Mg, w: NaHCO₃ is used at the following process steps across the biologics platforms it supports:
Cell & gene therapy
- Cell Collection & Isolation – Balanced salt handling.
Frequently asked questions
What is EBSS used for?
Washing and handling of cells; supports dissociation steps.
Does it contain Ca and Mg?
No – this variant is without Ca and Mg, with NaHCO₃.
In which grade is EBSS available?
It is available as a RUO product (P04-31500); a matching GMP grade is available on request.
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Related: Cell & Gene Therapy · Cell-Specific Media


