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Upstream & Downstream

DPBS (10x) w/o Ca & Mg, GMP grade

Process liquid / buffer · RUO (P04-53500) · GMP (CT-53500)

RUO · P04-53500
GMP · CT-53500
Upstream · USP
Downstream · DSP

DPBS (10x) without calcium and magnesium – a concentrated Dulbecco’s phosphate-buffered saline for economical preparation of wash, dilution and handling buffers.

Catalogue and GMP grade. DPBS (10x) w/o Ca & Mg is a RUO product (P04-53500); a matched GMP grade (CT-53500) is also available. Both are manufactured or assured by PAN-Celltech and released against a defined quality-control panel on every lot.

Why

  • Concentrated – 10x – dilute to working strength on site.
  • Without Ca & Mg – standard for wash and dissociation workflows.
  • Versatile – washing, dilution, buffer preparation.

Specification

Composition Dulbecco's PBS, 10x concentrate, without Ca & Mg
Form Concentrated solution
Grade RUO and GMP
Storage 2 °C to room temperature (15-25 °C)

Ordering

Grade Art. No. Pack size To Shop
RUO P04-53500 500 ml
GMP CT-53500 500 ml

Other pack sizes on request.

Request a quote
Beyond compendial – on request. Like every PAN process liquid, DPBS (10x) w/o Ca & Mg can be prepared with WFI Beyond – Water for Injection engineered beyond the Ph. Eur./USP monograph – for the most demanding endotoxin and purity requirements. Available on request.

In the process – by application (upstream & downstream)

DPBS (10x) w/o Ca & Mg is used at the following process steps across the biologics platforms it supports:

Monoclonal antibodies

  • Harvest & Clarification – 10x concentrate; dilute 1:10 with WFI to DPBS 1x. Harvest system buffer; membrane pre-wetting, equilibration and rinsing.
  • Size Exclusion Chromatography (SEC) – Polishing – SEC running-buffer prep: dilute 1:10 with WFI to DPBS 1x. 10x concentrate; shared with harvest (S5).

Cell & gene therapy

  • Ultrafiltration / Diafiltration – Concentrate to 1x.

Liposomes & LNP

  • Solvent removal & buffer exchange – 10x concentrate for large-volume, Ca/Mg-free diafiltration exchange buffer.

Frequently asked questions

In which grade is DPBS available?

It is available as a RUO product (P04-53500); a matching GMP grade (CT-53500) is also available.

Which processes is this product used in?

DPBS (10x) w/o Ca & Mg is used across the PAN monoclonal antibody, cell & gene therapy and liposome & LNP processes. See "In the process – by application (upstream & downstream)" above for the specific steps in each.

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