DNase I
Deoxyribonuclease I – degrades DNA to reduce viscosity. · Nuclease · RUO (P60-37780100)
DNase I cleaves single-stranded and double-stranded DNA (including chromatin) at neutral pH (optimum is a pH of 7.8), producing polynucleotides with a 5′-phosphate and a free hydroxyl-group in 3′ position. The activity and specificity (single-strand versus double-strand) of DNase I is determined by the surrounding ions. Maximum activation requires the presence of Mg2+ and Ca2+ ions. Magnesium ions mainly yield in the generation of single-strand nicks, while the presence of Mn2+ ions causes double-strand breaks.
PAN-Biotech quality. DNase I is manufactured by PAN-Biotech / PAN-Celltech and released against a defined quality-control panel.
Why
- DNA digestion – reduces viscosity.
- Cell preparation – single-cell suspensions.
- Residual DNA clearance – processing support.
- Research grade – RUO.
Specification
| Composition | Deoxyribonuclease I (DNase I) |
| Application | DNA digestion / viscosity reduction |
| Form | Enzyme |
| Grade | RUO |
| Storage | -20 °C |
Ordering
| Grade | Art. No. | Pack size |
|---|---|---|
| RUO | P60-37780100 | 100 mg |
Other pack sizes are available on request.
In the process – by application (upstream & downstream)
DNase I is used at the following process steps across the biologics platforms it supports:
Cell & gene therapy
- Nuclease Treatment – Complementary DNA digestion / viscosity reduction.
Vaccines
- Nuclease Treatment – Complementary DNA digestion.
mRNA
- DNase Digestion – Template DNA digestion / viscosity reduction.
Frequently asked questions
What is DNase I used for?
To degrade DNA and reduce viscosity in cell preparations and processing.
How is it stored?
At -20 °C.
In which grade is DNase I available?
It is available as a RUO product (P60-37780100); a matching GMP grade is available on request.
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Related: Cell & Gene Therapy · Cell-Specific Media