Citrate Buffer 100 mM, pH 3.0
Ready-to-use 100 mM citrate buffer at pH 3.0 for Protein A elution and low-pH viral inactivation. · GMP catalogue product · CT-62500
Citrate Buffer 100 mM, pH 3.0 is a defined, ready-to-use low-pH buffer with dual downstream use: as a Protein A affinity elution buffer and as the low-pH hold buffer for viral inactivation. With a fixed composition and pH, it is a general-purpose low-pH buffer used across monoclonal antibodies, vaccines, mRNA and cell & gene therapy.
Why
- Two steps, one buffer - Protein A elution and low-pH viral inactivation.
- Defined low pH - Fixed pH 3.0 ± 0.2.
- GMP grade - Catalogue GMP quality, released per lot.
- Multi-application - Applicable across antibody, vaccine and C&G downstream.
Specification
| Composition | 100 mM citrate |
| pH | 3.0 ± 0.2 |
| Form | Ready-to-use low-pH buffer |
| Storage | 15-25 °C (room temperature) |
| Grade | GMP |
Ordering
| Grade | Article No. | Pack size |
|---|---|---|
| GMP | CT-62500 | 500 ml |
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Other pack sizes are available on request.
In the downstream process - by application
Citrate Buffer 100 mM, pH 3.0 is used at the following process steps across the four biologics platforms:
Monoclonal antibodies
- Protein A capture - low-pH elution.
- Viral inactivation - low-pH hold buffer.
Vaccines
- Low-pH inactivation - acidification / hold.
- Affinity / IEX elution - acidic elution where used.
mRNA
- LNP formulation - low-pH mixing buffer for RNA + lipid nanoparticle assembly.
Cell & gene therapy
- Affinity capture (e.g. AAV) - low-pH elution.
- Viral inactivation - low-pH hold.
Frequently asked questions
What is Citrate Buffer 100 mM, pH 3.0 used for?
It is used as a Protein A elution buffer and as the low-pH hold buffer for viral inactivation in downstream processing.
What is the pH?
It is supplied at a fixed pH of 3.0 ± 0.2.
Is it only for antibodies?
No. It is a general low-pH buffer used across monoclonal antibodies, vaccines, mRNA and cell & gene therapy downstream.
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