MEM Eagle w: EBSS, w: L-Glutamine, w: 2.2 g/L NaHCO₃
Classic cell culture medium · RUO (P04-08500) · GMP (CT-08500)
Minimum Essential Medium (MEM), developed by Harry Eagle, is a widely used synthetic basal medium and an advancement of Basal Medium Eagle (BME). This formulation contains Earle’s Balanced Salt Solution (EBSS), L-glutamine, glucose and 2.2 g/L sodium bicarbonate, with phenol red and without HEPES. It supports a broad range of mammalian cells and is readily supplemented with amino acids or small amounts of serum.
One GMP quality. MEM Eagle w: EBSS, w: L-Glutamine, w: 2.2 g/L NaHCO₃ is a GMP-grade catalogue product (CT-08500), manufactured or assured by PAN-Celltech and released against a defined quality-control panel on every lot.
Why
- Widely used synthetic medium – one of the most-used base media for mammalian cells.
- EBSS-buffered – with L-glutamine and 2.2 g/L sodium bicarbonate.
- Adherent-cell friendly – suitable for virus propagation on cell substrates.
Specification
| Base | MEM Eagle (Minimum Essential Medium) with Earle's Balanced Salt Solution (EBSS) |
| Key features | With L-glutamine; 2.2 g/L NaHCO₃ |
| Form | Liquid medium |
| Grade | RUO & GMP |
| Storage | 2-8 °C, protected from light |
Ordering
In the process – by application (upstream & downstream)
MEM Eagle w: EBSS, w: L-Glutamine, w: 2.2 g/L NaHCO₃ is used at the following process steps across the biologics platforms it supports:
Cell & gene therapy
- Vector Production / Transfection – Adherent producer-cell substrate for viral vector production.
Vaccines
- Cell-line-specific viral culture media
Frequently asked questions
Which processes is this product used in?
MEM Eagle w: EBSS, w: L-Glutamine, w: 2.2 g/L NaHCO₃ is used across the PAN Monoclonal antibody and Cell & gene therapy processes. See the section above for the specific steps in each.
In which grade is MEM Eagle available?
It is available as a RUO product (P04-08500); a matching GMP grade (CT-08500) is also available.
One partner across every application. Explore the full GMP-grade catalogue or use the Smart Product Search.


