William’s Medium E
William’s Medium E – Classic Medium for Hepatocyte / Liver Cell Culture
William’s Medium E, classic cell culture medium
RUO (P04-29050)
William’s Medium E is used for the long-term cultivation of adult rat liver epithelial cells and can also be used for other mammalian cells. It is usually supplemented with 5-10 % FBS to achieve long-term cultivation. It is offered in several configurations differing in glutamine form and phenol red.
Classic William’s Medium E for long-term liver-cell culture. A well-established medium for hepatocytes and adult rat liver epithelial cells, typically with 5-10 % FBS.
Why William’s Medium E
- Classic formulation – the established William’s Medium E recipe for reliable, reproducible culture.
- Glutamine options – available as with stable glutamine, glutamine-free.
- Glucose choice – standard and high-glucose options for different energy demands.
- Broad use – suitable for a wide range of routine cell culture applications.
Formulation at a glance
| Type | Classic basal medium (William’s E) |
| Developed for | Long-term culture of adult rat liver epithelial cells |
| Typical supplementation | 5-10 % FBS for long-term cultivation |
| Buffer | 2.24 g/L NaHCO3 |
| Format | Liquid, sterile-filtered |
| Storage | +2 °C to +8 °C (stability 2 years) |
Ordering & grades
| Grade | Article No. | Glucose | L-Glutamine | Other | Size | Use | To Shop |
|---|---|---|---|---|---|---|---|
| RUO | P04-29050S4 | w/o | w/o | w/o phenol red | 500 ml | Research use | |
| RUO | P04-29050 | 2.0 g/L | w/o | 500 ml | Research use | ||
| RUO | P04-29510 | 2.0 g/L | w/o | w/o phenol red | 500 ml | Research use | |
| RUO | P04-29150 | 2.0 g/L | stable | 500 ml | Research use | ||
| RUO | P04-29510S1 | 2.0 g/L | stable | w/o phenol red | 500 ml | Research use |
Applications
- Long-term culture of adult rat liver epithelial cells
- Hepatocyte culture
- General mammalian cell culture with 5-10% FBS
Frequently asked questions
What is William's Medium E used for?
The long-term cultivation of adult rat liver epithelial cells; it can also be used for other mammalian cells, usually supplemented with 5-10 % FBS.
Which configurations are available?
With L-glutamine, with stable glutamine, or glutamine-free, with phenol-red-free options.
How is it stored?
William’s Medium E is stored at +2 °C to +8 °C, with a stability of 2 years from date of production.
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