Trypsin 0.25 % / 1 mM EDTA 4 Na in DPBS, w/o: Ca and Mg
Trypsin 0.25 % / 1 mM EDTA 4 Na in DPBS, w/o: Ca and Mg – Trypsin Cell Detachment Solution
Trypsin 0.25 % / 1 mM EDTA 4 Na in DPBS, w/o: Ca and Mg, ready-to-use trypsin cell detachment solution
RUO (P10-028100)
Trypsin 0.25 % / 1 mM EDTA 4 Na in DPBS, w/o: Ca and Mg is a ready-to-use cell detachment solution containing 0.25 % trypsin and 1 mM EDTA (4 Na), formulated in DPBS without calcium and magnesium. Trypsin is a porcine pancreas-derived enzyme (1:250) that dissociates anchorage-dependent cells; EDTA is added to chelate calcium and magnesium and enhance trypsin activity. This variant uses the tetrasodium salt form of EDTA (EDTA 4 Na). After detachment, trypsin should be neutralised with serum-containing medium or a trypsin inhibitor.
Reliable trypsin detachment for routine passaging. 0.25 % trypsin with 1 mM EDTA (4 Na) in DPBS, Ca/Mg-free for effective dissociation.
Why Trypsin 0.25 % / 1 mM EDTA 4 Na in DPBS, w/o: Ca and Mg
- Effective dissociation – porcine trypsin (1:250) for anchorage-dependent cells.
- EDTA-enhanced – EDTA chelates Ca/Mg to boost trypsin activity.
- DPBS base, Ca/Mg-free – formulated to support efficient trypsinisation.
- Neutralise after use – stop with serum-containing medium or trypsin inhibitor.
Formulation at a glance
| Type | Cell detachment solution |
| Trypsin | 0.25 % (1:250, porcine) |
| EDTA | 1 mM EDTA (4 Na) |
| Base | DPBS, w/o Ca and Mg |
| Storage | -20 °C |
Ordering & grades
| Grade | Article No. | Size | Use | To Shop |
|---|---|---|---|---|
| RUO | P10-028100 | 100 ml | Research use |
Applications
- Routine detachment and passaging of adherent cells
- Subculture of anchorage-dependent cell lines
Frequently asked questions
What is Trypsin 0.25 % / 1 mM EDTA 4 Na in DPBS, w/o: Ca and Mg?
Trypsin 0.25 % / 1 mM EDTA 4 Na in DPBS, w/o: Ca and Mg is a ready-to-use cell detachment solution containing 0.25 % trypsin and 1 mM EDTA (4 Na), formulated in DPBS without calcium and magnesium. Trypsin is a porcine pancreas-derived enzyme (1:250) that dissociates anchorage-dependent cells; EDTA is added to chelate calcium and magnesium and enhance trypsin activity. This variant uses the tetrasodium salt form of EDTA (EDTA 4 Na). After detachment, trypsin should be neutralised with serum-containing medium or a trypsin inhibitor.
How is Trypsin 0.25 % / 1 mM EDTA 4 Na in DPBS, w/o: Ca and Mg used?
Aspirate medium, rinse the monolayer with Ca/Mg-free salt solution, add enough pre-warmed trypsin to cover the cells and incubate until they round up and detach; then neutralise, centrifuge and resuspend in fresh medium.
How is Trypsin 0.25 % / 1 mM EDTA 4 Na in DPBS, w/o: Ca and Mg stored?
Store the solution at -20 °C. Stable 2 years from production.
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