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Pancoll rat, density 1.091 g/ml

Pancoll rat, density 1.091 g/ml – Pancoll Density Gradient Separating Solution

Pancoll rat, density 1.091 g/ml, density gradient separating solution (1.091 g/ml)
RUO (P04-65500)

Density gradient medium
RUO · P04-65500
For rat blood cells

Pancoll rat, density 1.091 g/ml is a ready-to-use density gradient separation solution based on a hydrophilic polysaccharide (molecular weight 400,000 Da). At a density of 1.091 g/ml, it is optimised for isolation of mononuclear cells from rat blood by density gradient centrifugation: cells are layered over the Pancoll solution and, after centrifugation, fractions separate according to their density while red cells and granulocytes aggregate and sediment. The density and osmolality are optimised for rat blood.

Reliable density-gradient separation. A ready-to-use Pancoll solution at 1.091 g/ml for isolation of mononuclear cells from rat blood.

Why Pancoll rat, density 1.091 g/ml

  • Defined density – 1.091 g/ml, matched to the target fraction.
  • Ready-to-use – no preparation, layer and centrifuge.
  • Gentle separation – hydrophilic polysaccharide, well tolerated by cells.
  • Reproducible – quality-controlled for consistent gradients.

Formulation at a glance

Type Density gradient medium
Density 1.091 g/ml
Polymer Polysaccharide, MW 400,000 Da
Format Liquid, ready-to-use
Storage +2 °C to +20 °C, in the dark

Ordering & grades

Grade Article No. Size Use To Shop
RUO P04-65100 100 ml Research use
RUO P04-65500 500 ml Research use

 

Applications

  • Isolation of mononuclear cells from rat blood
  • Density gradient centrifugation of blood cells

Frequently asked questions

What is Pancoll rat, density 1.091 g/ml?

Pancoll rat, density 1.091 g/ml is a ready-to-use density gradient separation solution based on a hydrophilic polysaccharide (molecular weight 400,000 Da). At a density of 1.091 g/ml, it is optimised for isolation of mononuclear cells from rat blood by density gradient centrifugation: cells are layered over the Pancoll solution and, after centrifugation, fractions separate according to their density while red cells and granulocytes aggregate and sediment. The density and osmolality are optimised for rat blood.

How is Pancoll rat, density 1.091 g/ml used?

Layer the diluted blood sample carefully over the Pancoll solution without mixing, centrifuge (e.g. 800 g, 20 min, 20 °C, brake off), then harvest the target cell band and wash.

How is Pancoll rat, density 1.091 g/ml stored?

Store at +2 °C to +20 °C in the dark. Stable 3 years from production.

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