Pancoll human, density 1.077 g/ml
Pancoll human, density 1.077 g/ml – Pancoll Density Gradient Separating Solution
Pancoll human, density 1.077 g/ml, density gradient separating solution (1.077 g/ml)
RUO (P04-60100)
Pancoll human, density 1.077 g/ml is a ready-to-use density gradient separation solution based on a hydrophilic polysaccharide (molecular weight 400,000 Da). At a density of 1.077 g/ml, it is optimised for isolation of human peripheral blood mononuclear cells (lymphocytes and monocytes) by density gradient centrifugation: cells are layered over the Pancoll solution and, after centrifugation, fractions separate according to their density while red cells and granulocytes aggregate and sediment. With Pancoll human 1.077, lymphocytes, monocytes and platelets are recovered above the Pancoll layer while erythrocytes and granulocytes sediment.
Reliable density-gradient separation. A ready-to-use Pancoll solution at 1.077 g/ml for isolation of human peripheral blood mononuclear cells (lymphocytes and monocytes).
Why Pancoll human, density 1.077 g/ml
- Defined density – 1.077 g/ml, matched to the target fraction.
- Ready-to-use – no preparation, layer and centrifuge.
- Gentle separation – hydrophilic polysaccharide, well tolerated by cells.
- Reproducible – quality-controlled for consistent gradients.
Formulation at a glance
| Type | Density gradient medium |
| Density | 1.077 g/ml |
| Polymer | Polysaccharide, MW 400,000 Da |
| Format | Liquid, ready-to-use |
| Storage | +2 °C to +20 °C, in the dark |
Ordering & grades
| Grade | Article No. | Size | Use | To Shop |
|---|---|---|---|---|
| RUO | P04-60100 | 100 ml | Research use | |
| RUO | P04-60500 | 500 ml | Research use | |
| RUO | P04-601000 | 1000 ml | Research use | |
| RUO | P04-605000 | 5 L | Research use |
Applications
- Isolation of human peripheral blood mononuclear cells (lymphocytes and monocytes)
- Density gradient centrifugation of blood cells
Frequently asked questions
What is Pancoll human, density 1.077 g/ml?
Pancoll human, density 1.077 g/ml is a ready-to-use density gradient separation solution based on a hydrophilic polysaccharide (molecular weight 400,000 Da). At a density of 1.077 g/ml, it is optimised for isolation of human peripheral blood mononuclear cells (lymphocytes and monocytes) by density gradient centrifugation: cells are layered over the Pancoll solution and, after centrifugation, fractions separate according to their density while red cells and granulocytes aggregate and sediment. With Pancoll human 1.077, lymphocytes, monocytes and platelets are recovered above the Pancoll layer while erythrocytes and granulocytes sediment.
How is Pancoll human, density 1.077 g/ml used?
Layer the diluted blood sample carefully over the Pancoll solution without mixing, centrifuge (e.g. 800 g, 20 min, 20 °C, brake off), then harvest the target cell band and wash.
How is Pancoll human, density 1.077 g/ml stored?
Store at +2 °C to +20 °C in the dark. Stable 3 years from production.
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