HybridBoost
Serum-Free Hybridoma Medium
Defined, serum-free medium for high-performance hybridoma and myeloma culture
RUO (P04-995905) · GMP grade on request (CT-995905)
HybridBoost is an innovative defined, serum-free medium for high-performance hybridoma cultures, featuring stable glutamine, recombinant human insulin, and no added hydrolysates. Its built-in surfactant supports agitated systems, while animal-derived components and proteins are each <0.1 % w/v, supporting tight process control. For cholesterol-dependent lines, supplementation is recommended, and adaptation protocols are provided.
One product, two matched grades. HybridBoost is available as a research-use medium (P04-995910) and – like every PAN-Biotech product – in GMP quality on request (matched grade CT-995905). The same formulation carries you from research through further manufacturing to GMP with one partner.
Why HybridBoost
- Defined & serum-free – no fetal bovine serum, removing a key source of lot-to-lot variability and regulatory risk.
- No added hydrolysates – a chemically leaner background that supports tight process control and cleaner downstream purification.
- Stable glutamine – reduced ammonia build-up and more consistent performance over extended culture.
- Recombinant human insulin – animal-origin-conscious growth support.
- Built-in surfactant – shear protection for shaken, stirred and agitated suspension systems.
- < 0.1% w/v animal-derived components & proteins – for a controlled background and monoclonal antibody yield.
Formulation at a glance
| Property | Detail |
|---|---|
| Cell type | Hybridoma / myeloma cells |
| Classification | Serum-free, cholesterol-free, defined |
| Glutamine | Stable glutamine |
| Insulin | Recombinant human insulin |
| Phenol red | Without phenol red |
| Sodium bicarbonate | 2.438 g/L NaHCO3 |
| Hydrolysates | None added |
| Animal-derived components | < 0.1% w/v |
| Proteins | < 0.1% w/v |
| Surfactant | Built-in (agitation / shear protection) |
Cholesterol-dependent lines: supplementation recommended; adaptation protocols available on request.
Ordering & grades
Applications
- Monoclonal antibody production in hybridoma and myeloma lines
- Suspension and agitated culture (shaken flasks, stirred / wave systems)
- Serum-free adaptation of established hybridoma clones
- Process development where a defined, hydrolysate-free background is required
Frequently asked questions
Is HybridBoost serum-free?
Yes. HybridBoost is a defined, serum-free medium developed for hybridoma cultures. It contains no fetal bovine serum and no added hydrolysates; animal-derived components and proteins are each <0.1% w/v.
Is HybridBoost available in GMP quality?
Yes. Every PAN-Biotech product is available in GMP quality on request. The matched GMP grade of HybridBoost is CT-995905, manufactured GMP EU Annex 1–compliant by PAN-Celltech. Request the GMP grade here.
Do I need to add cholesterol?
HybridBoost is cholesterol-free. For cholesterol-dependent hybridoma lines, cholesterol supplementation is recommended; adaptation protocols are provided on request. For cholesterol-containing defined media, see Panserin H8000 (P04-718000).
Which catalogue number should I order?
For research use, order HybridBoost P04-995910 (1000 ml). For GMP-compliant supply, request the matched grade CT-995905 (500 ml).
One partner, from research through further manufacturing to GMP. Explore the full GMP-grade catalogue or use the Smart Product Search to find a matched medium for your cell line.
Related: ISF-1 Hybridoma Medium (P04-995968) · Panserin H8000 (P04-718000) · GMP-Grade Products