EDTA 1 % in DPBS, w/o Ca & Mg
EDTA 1 % in DPBS – Non-Enzymatic Cell Dissociation
EDTA 1 % in DPBS, w/o Ca & Mg, chelating agent for mild non-enzymatic detachment
RUO (P10-026100)
EDTA 1 % in DPBS without calcium and magnesium is a chelating solution for mild, non-enzymatic detachment of adherent cells. EDTA binds divalent cations that support cell-to-cell and cell-substrate adhesion, loosening cells from the surface without proteolytic enzymes. It is useful for cells that are sensitive to trypsin, for gentle dissociation of colonies, and as a wash to weaken adhesion before or instead of enzymatic treatment.
Enzyme-free, gentle dissociation. A 1 % EDTA solution in Ca/Mg-free DPBS for detaching trypsin-sensitive cells.
Why EDTA 1 % in DPBS, w/o Ca & Mg
- Non-enzymatic – chelates divalent cations without proteolysis.
- Gentle – suited to trypsin-sensitive cells and colonies.
- Ca/Mg-free base – DPBS without divalent cations enhances the effect.
- Ready-to-use – 1 % solution, sterile.
Formulation at a glance
| Type | Non-enzymatic dissociation reagent |
| Concentration | 1 % EDTA |
| Base | DPBS, w/o Ca and Mg |
| Format | Liquid, sterile |
| Storage | +2 °C to +8 °C |
Ordering & grades
| Grade | Article No. | Size | Use | To Shop |
|---|---|---|---|---|
| RUO | P10-026100 | 100 ml | Research use | |
| RUO | P10-026500 | 500 ml | Research use |
Applications
- Non-enzymatic detachment of adherent cells
- Gentle dissociation of trypsin-sensitive cells
Frequently asked questions
What is EDTA 1 % in DPBS, w/o Ca & Mg?
EDTA 1 % in DPBS without calcium and magnesium is a chelating solution for mild, non-enzymatic detachment of adherent cells. EDTA binds divalent cations that support cell-to-cell and cell-substrate adhesion, loosening cells from the surface without proteolytic enzymes. It is useful for cells that are sensitive to trypsin, for gentle dissociation of colonies, and as a wash to weaken adhesion before or instead of enzymatic treatment.
How is EDTA 1 % in DPBS, w/o Ca & Mg used?
Aspirate medium, rinse the monolayer, then add EDTA solution and incubate until cells loosen; collect in medium.
How is EDTA 1 % in DPBS, w/o Ca & Mg stored?
Store at +2 °C to +8 °C.
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