EBSS, w/o: Ca and Mg, w: 2.2 g/L NaHCO3
EBSS w/o Ca and Mg, w: 2.2 g/L NaHCO3 – Earle’s Balanced Salt Solution
EBSS, w/o: Ca and Mg, w: 2.2 g/L NaHCO3 – Earle’s Balanced Salt Solution
RUO (P04-31500) · GMP grade on request
Earle’s Balanced Salt Solution (EBSS) without calcium and magnesium and with 2.2 g/L sodium bicarbonate is a balanced salt solution that maintains pH and osmolality and provides essential inorganic ions. Being free of calcium and magnesium, it is suited to washing and handling steps in dissociation workflows, and it is supplied with bicarbonate for use under CO2 buffering.
Bicarbonate-buffered Earle’s salts, Ca/Mg-free. A balanced salt solution for dissociation-compatible washing and CO2 buffering.
Why EBSS, w/o: Ca and Mg, w: 2.2 g/L NaHCO3
- Ca/Mg-free – compatible with dissociation workflows.
- With bicarbonate – contains 2.2 g/L NaHCO3 for CO2 buffering.
- Balanced salts – maintains pH and osmolality, provides inorganic ions.
- Quality controlled – sterile and released to specifications.
Formulation at a glance
| Type | Balanced salt solution |
| Product | EBSS (Earle’s) |
| Ions | w/o Ca and Mg, w: 2.2 g/L NaHCO3 |
| Format | Liquid, sterile |
| Storage | +2 °C to room temperature |
Ordering & grades
| Grade | Article No. | Size | Use | To Shop |
|---|---|---|---|---|
| RUO | P04-31500 | 500 ml | Research use |
Applications
- Washing and handling in dissociation workflows
- Base for media and reagent preparation
Frequently asked questions
What is EBSS, w/o: Ca and Mg, w: 2.2 g/L NaHCO3?
Earle’s Balanced Salt Solution (EBSS) without calcium and magnesium and with 2.2 g/L sodium bicarbonate is a balanced salt solution that maintains pH and osmolality and provides essential inorganic ions. Being free of calcium and magnesium, it is suited to washing and handling steps in dissociation workflows, and it is supplied with bicarbonate for use under CO2 buffering.
How is EBSS, w/o: Ca and Mg, w: 2.2 g/L NaHCO3 used?
Use as supplied for washing and handling; suited to CO2-buffered conditions.
How is EBSS, w/o: Ca and Mg, w: 2.2 g/L NaHCO3 stored?
Store at +2 °C to room temperature.
Is a GMP-grade version available?
GMP grade is available on request for PAN-Biotech catalogue products (excluding sera), manufactured GMP EU Annex 1-compliant by PAN-Celltech.
One partner, from research through further manufacturing to GMP. Explore the full GMP-grade catalogue or use the Smart Product Search to find a matched product for your cell line.
Related: EBSS, w: Ca and Mg, w/o: NaHCO3 · HBSS, w/o: Ca and Mg, w: 0.35 g/L NaHCO3, w: Phenol red · GMP-Grade Products


