EBSS, w: Ca and Mg, w/o: NaHCO3
EBSS w: Ca and Mg, w/o NaHCO3 – Earle’s Balanced Salt Solution
EBSS, w: Ca and Mg, w/o: NaHCO3 – Earle’s Balanced Salt Solution for washing and handling
RUO (P04-30500) · GMP grade on request
Earle’s Balanced Salt Solution (EBSS) with calcium and magnesium and without sodium bicarbonate is a balanced salt solution that maintains pH and osmolality and provides essential inorganic ions. EBSS is a classic salt solution used to wash and handle cells and tissues and as a base for media and reagents. This variant contains the divalent cations Ca and Mg and is supplied without added bicarbonate.
Classic Earle’s salts for the culture lab. A balanced salt solution with Ca and Mg for washing, handling and reagent preparation.
Why EBSS, w: Ca and Mg, w/o: NaHCO3
- Balanced salts – maintains pH and osmolality, provides inorganic ions.
- Contains Ca and Mg – divalent cations for adhesion-dependent steps.
- Without bicarbonate – add bicarbonate or buffer as your protocol requires.
- Quality controlled – sterile and released to specifications.
Formulation at a glance
| Type | Balanced salt solution |
| Product | EBSS (Earle’s) |
| Ions | With Ca and Mg, w/o NaHCO3 |
| Format | Liquid, sterile |
| Storage | +2 °C to room temperature |
Ordering & grades
| Grade | Article No. | Size | Use | To Shop |
|---|---|---|---|---|
| RUO | P04-30500 | 500 ml | Research use |
Applications
- Washing and handling of cells and tissues
- Base for media and reagent preparation
Frequently asked questions
What is EBSS, w: Ca and Mg, w/o: NaHCO3?
Earle’s Balanced Salt Solution (EBSS) with calcium and magnesium and without sodium bicarbonate is a balanced salt solution that maintains pH and osmolality and provides essential inorganic ions. EBSS is a classic salt solution used to wash and handle cells and tissues and as a base for media and reagents. This variant contains the divalent cations Ca and Mg and is supplied without added bicarbonate.
How is EBSS, w: Ca and Mg, w/o: NaHCO3 used?
Use as supplied for washing and handling; add bicarbonate or buffer according to your protocol.
How is EBSS, w: Ca and Mg, w/o: NaHCO3 stored?
Store at +2 °C to room temperature.
Is a GMP-grade version available?
GMP grade is available on request for PAN-Biotech catalogue products (excluding sera), manufactured GMP EU Annex 1-compliant by PAN-Celltech.
One partner, from research through further manufacturing to GMP. Explore the full GMP-grade catalogue or use the Smart Product Search to find a matched product for your cell line.
Related: EBSS, w/o: Ca and Mg, w: 2.2 g/L NaHCO3 · HBSS, w: Ca and Mg, w: 0.35 g/L NaHCO3 · GMP-Grade Products