Universal Electroporation / Nucleofection Buffer
Buffer for electroporation- and nucleofection-based delivery of nucleic acids into cells. · Electroporation / nucleofection buffer · GMP (CT-34100)
The Universal Electroporation / Nucleofection Buffer is a defined buffer for electroporation- and nucleofection-based delivery of nucleic acids – plasmid DNA, mRNA or ribonucleoprotein (RNP) complexes – into mammalian cells. It is designed to support efficient, low-toxicity transfection of hard-to-transfect primary cells such as T cells, NK cells and stem cells in cell and gene therapy workflows. It will be available as a GMP-grade (CT) catalogue product (Article Number CT-34100).
One GMP quality. Universal Electroporation / Nucleofection Buffer is a GMP-grade catalogue product, manufactured by PAN-Celltech under EU GMP Annex 1 and released against a defined quality-control panel on every lot. Article Number CT-34100.
Why
- Universal – for a broad range of primary and established cell types.
- Low toxicity – gentle conditions to preserve cell viability.
- DNA / RNA / RNP – compatible with plasmid, mRNA and RNP delivery.
- GMP grade – manufactured to EU GMP Annex 1.
Specification
| Composition | Phosphate buffer (pH 7.2) with KCl, MgCl₂ and D-mannitol |
| Application | Electroporation / nucleofection |
| Form | Solution |
| Appearance | clear, colourless solution |
| Grade | GMP |
| Storage | +2 to +8 °C |
Ordering
| Grade | Art. No. | Pack size |
|---|---|---|
| GMP | CT-34100 | 100 mL |
GMP Article Number: CT-34100. Further pack sizes on request.
In the process – by application (upstream & downstream)
Universal Electroporation / Nucleofection Buffer is used at the following process steps across the biologics platforms it supports:
Cell & gene therapy
- Genetic modification / Transduction – Low-conductivity electroporation/nucleofection buffer – PAN product on request; for non-viral gene modification.
Frequently asked questions
What is the Universal Electroporation / Nucleofection Buffer used for?
It is used as the buffer medium for electroporation- or nucleofection-based delivery of DNA, mRNA or RNP complexes into cells during ex-vivo gene modification.
In which grade is it available?
It is available as a GMP-grade product; Article Number CT-34100.
One partner across every application. PAN-Biotech products support cell & gene therapy, monoclonal antibodies, vaccines and mRNA – explore the full GMP-grade catalogue or use the Smart Product Search.
Related: Cell & gene therapy