IVT Reaction Buffer 10x
10x concentrated reaction buffer for in-vitro transcription (IVT) of mRNA. · IVT reaction buffer · GMP (CT-14100)
The IVT Reaction Buffer (10x) is a concentrated reaction buffer for in-vitro transcription (IVT) of messenger RNA from a linearised DNA template. It provides the buffered reaction environment for RNA-polymerase-driven transcription during cell-free mRNA synthesis. It will be available as a GMP-grade (CT) catalogue product (Article Number CT-14100).
One GMP quality. IVT Reaction Buffer 10x is a GMP-grade catalogue product, manufactured by PAN-Celltech under EU GMP Annex 1 and released against a defined quality-control panel on every lot. Article Number CT-14100.
Why
- For IVT – buffered environment for RNA-polymerase transcription.
- 10x concentrate – convenient dilution into the reaction.
- mRNA synthesis – core reagent of the cell-free IVT step.
- GMP grade – manufactured to EU GMP Annex 1.
Specification
| Composition | Tris-HCl buffer (pH 7.9) with MgCl₂, spermidine and DTT |
| Application | In-vitro transcription (IVT) |
| Concentration | 10x |
| Form | Solution |
| Appearance | clear, colourless to slightly yellowish solution |
| Grade | GMP |
| Storage | -20 °C |
Ordering
| Grade | Art. No. | Pack size |
|---|---|---|
| GMP | CT-14100 | 100 mL |
GMP Article Number: CT-14100. Further pack sizes on request.
Mg²⁺ note: In the final IVT reaction, adjust the Mg²⁺ concentration to the NTPs used – keep total Mg²⁺ above total NTP.
In the process – by application (upstream & downstream)
IVT Reaction Buffer 10x is used at the following process steps across the biologics platforms it supports:
Cell & gene therapy
- Genetic modification / payload – IVT of mRNA / guide RNA (transient CAR mRNA, CRISPR gRNA).
mRNA
- In Vitro Transcription (IVT) – Ready-to-use transcription buffer – PAN product on request. PAN already supplies the components.
Frequently asked questions
What is the IVT Reaction Buffer 10x used for?
It provides the buffered reaction environment for RNA-polymerase-driven in-vitro transcription (IVT) of mRNA from a DNA template.
In which grade is it available?
It is available as a GMP-grade product; Article Number CT-14100.
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Related: mRNA