Pancoll mouse, density 1.086 g/ml
Pancoll mouse, density 1.086 g/ml – Pancoll Density Gradient Separating Solution
Pancoll mouse, density 1.086 g/ml, density gradient separating solution (1.086 g/ml)
RUO (P04-64500)
Pancoll mouse, density 1.086 g/ml is a ready-to-use density gradient separation solution based on a hydrophilic polysaccharide (molecular weight 400,000 Da). At a density of 1.086 g/ml, it is optimised for isolation of mononuclear cells from mouse blood by density gradient centrifugation: cells are layered over the Pancoll solution and, after centrifugation, fractions separate according to their density while red cells and granulocytes aggregate and sediment. The density and osmolality are optimised for murine blood.
Reliable density-gradient separation. A ready-to-use Pancoll solution at 1.086 g/ml for isolation of mononuclear cells from mouse blood.
Why Pancoll mouse, density 1.086 g/ml
- Defined density – 1.086 g/ml, matched to the target fraction.
- Ready-to-use – no preparation, layer and centrifuge.
- Gentle separation – hydrophilic polysaccharide, well tolerated by cells.
- Reproducible – quality-controlled for consistent gradients.
Formulation at a glance
| Type | Density gradient medium |
| Density | 1.086 g/ml |
| Polymer | Polysaccharide, MW 400,000 Da |
| Format | Liquid, ready-to-use |
| Storage | +2 °C to +20 °C, in the dark |
Ordering & grades
Applications
- Isolation of mononuclear cells from mouse blood
- Density gradient centrifugation of blood cells
Frequently asked questions
What is Pancoll mouse, density 1.086 g/ml?
Pancoll mouse, density 1.086 g/ml is a ready-to-use density gradient separation solution based on a hydrophilic polysaccharide (molecular weight 400,000 Da). At a density of 1.086 g/ml, it is optimised for isolation of mononuclear cells from mouse blood by density gradient centrifugation: cells are layered over the Pancoll solution and, after centrifugation, fractions separate according to their density while red cells and granulocytes aggregate and sediment. The density and osmolality are optimised for murine blood.
How is Pancoll mouse, density 1.086 g/ml used?
Layer the diluted blood sample carefully over the Pancoll solution without mixing, centrifuge (e.g. 800 g, 20 min, 20 °C, brake off), then harvest the target cell band and wash.
How is Pancoll mouse, density 1.086 g/ml stored?
Store at +2 °C to +20 °C in the dark. Stable 3 years from production.
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