Trypsin / EDTA 0.05 % / 0.02 % in DPBS, with Phenol red
Trypsin / EDTA 0.05 % / 0.02 % in DPBS, with Phenol red – Trypsin Cell Detachment Solution
Trypsin / EDTA 0.05 % / 0.02 % in DPBS, with Phenol red, ready-to-use trypsin cell detachment solution
RUO (P10-0231SP)
Trypsin / EDTA 0.05 % / 0.02 % in DPBS, with Phenol red is a ready-to-use cell detachment solution containing 0.05 % trypsin and 0.02 % EDTA, formulated in DPBS without calcium and magnesium, with phenol red. Trypsin is a porcine pancreas-derived enzyme (1:250) that dissociates anchorage-dependent cells; EDTA is added to chelate calcium and magnesium and enhance trypsin activity. A low-concentration trypsin-EDTA with phenol red for gentle routine passaging. A GMP-grade version (CT-023100, without phenol red) is available. After detachment, trypsin should be neutralised with serum-containing medium or a trypsin inhibitor.
Reliable trypsin detachment for routine passaging. 0.05 % trypsin with 0.02 % EDTA in DPBS, Ca/Mg-free for effective dissociation.
Why Trypsin / EDTA 0.05 % / 0.02 % in DPBS, with Phenol red
- Effective dissociation – porcine trypsin (1:250) for anchorage-dependent cells.
- EDTA-enhanced – EDTA chelates Ca/Mg to boost trypsin activity.
- DPBS base, Ca/Mg-free – formulated to support efficient trypsinisation.
- Neutralise after use – stop with serum-containing medium or trypsin inhibitor.
Formulation at a glance
| Type | Cell detachment solution |
| Trypsin | 0.05 % (1:250, porcine) |
| EDTA | 0.02 % EDTA |
| Base | DPBS, w/o Ca and Mg, with phenol red |
| Storage | -20 °C |
Ordering & grades
| Grade | Article No. | Size | Use | To Shop |
|---|---|---|---|---|
| RUO | P10-0231SP | 100 ml | Research use | |
| RUO | P10-0235SP | 500 ml | Research use | |
| GMP | CT-023100 | 100 ml | GMP grade |
Applications
- Routine detachment and passaging of adherent cells
- Subculture of anchorage-dependent cell lines
Frequently asked questions
What is Trypsin / EDTA 0.05 % / 0.02 % in DPBS, with Phenol red?
Trypsin / EDTA 0.05 % / 0.02 % in DPBS, with Phenol red is a ready-to-use cell detachment solution containing 0.05 % trypsin and 0.02 % EDTA, formulated in DPBS without calcium and magnesium, with phenol red. Trypsin is a porcine pancreas-derived enzyme (1:250) that dissociates anchorage-dependent cells; EDTA is added to chelate calcium and magnesium and enhance trypsin activity. A low-concentration trypsin-EDTA with phenol red for gentle routine passaging. A GMP-grade version (CT-023100, without phenol red) is available. After detachment, trypsin should be neutralised with serum-containing medium or a trypsin inhibitor.
How is Trypsin / EDTA 0.05 % / 0.02 % in DPBS, with Phenol red used?
Aspirate medium, rinse the monolayer with Ca/Mg-free salt solution, add enough pre-warmed trypsin to cover the cells and incubate until they round up and detach; then neutralise, centrifuge and resuspend in fresh medium.
How is Trypsin / EDTA 0.05 % / 0.02 % in DPBS, with Phenol red stored?
Store the solution at -20 °C. Stable 2 years from production.
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