Trypsin 0.25 % / 1 mM EDTA 4 Na in HBSS, w/o: Ca and Mg, with Phenol red
Trypsin 0.25 % / 1 mM EDTA 4 Na in HBSS, w/o: Ca and Mg, with Phenol red – Trypsin Cell Detachment Solution
Trypsin 0.25 % / 1 mM EDTA 4 Na in HBSS, w/o: Ca and Mg, with Phenol red, ready-to-use trypsin cell detachment solution
RUO (P10-029100)
Trypsin 0.25 % / 1 mM EDTA 4 Na in HBSS, w/o: Ca and Mg, with Phenol red is a ready-to-use cell detachment solution containing 0.25 % trypsin and 1 mM EDTA (4 Na), formulated in HBSS without calcium and magnesium, with phenol red. Trypsin is a porcine pancreas-derived enzyme (1:250) that dissociates anchorage-dependent cells; EDTA is added to chelate calcium and magnesium and enhance trypsin activity. It is formulated on an HBSS base with the tetrasodium EDTA salt and phenol red indicator. After detachment, trypsin should be neutralised with serum-containing medium or a trypsin inhibitor.
Reliable trypsin detachment for routine passaging. 0.25 % trypsin with 1 mM EDTA (4 Na) in HBSS, Ca/Mg-free for effective dissociation.
Why Trypsin 0.25 % / 1 mM EDTA 4 Na in HBSS, w/o: Ca and Mg, with Phenol red
- Effective dissociation – porcine trypsin (1:250) for anchorage-dependent cells.
- EDTA-enhanced – EDTA chelates Ca/Mg to boost trypsin activity.
- HBSS base, Ca/Mg-free – formulated to support efficient trypsinisation.
- Neutralise after use – stop with serum-containing medium or trypsin inhibitor.
Formulation at a glance
| Type | Cell detachment solution |
| Trypsin | 0.25 % (1:250, porcine) |
| EDTA | 1 mM EDTA (4 Na) |
| Base | HBSS, w/o Ca and Mg, with phenol red |
| Storage | -20 °C |
Ordering & grades
| Grade | Article No. | Size | Use | To Shop |
|---|---|---|---|---|
| RUO | P10-029100 | 100 ml | Research use | |
| RUO | P10-029500 | 500 ml | Research use |
Applications
- Routine detachment and passaging of adherent cells
- Subculture of anchorage-dependent cell lines
Frequently asked questions
What is Trypsin 0.25 % / 1 mM EDTA 4 Na in HBSS, w/o: Ca and Mg, with Phenol red?
Trypsin 0.25 % / 1 mM EDTA 4 Na in HBSS, w/o: Ca and Mg, with Phenol red is a ready-to-use cell detachment solution containing 0.25 % trypsin and 1 mM EDTA (4 Na), formulated in HBSS without calcium and magnesium, with phenol red. Trypsin is a porcine pancreas-derived enzyme (1:250) that dissociates anchorage-dependent cells; EDTA is added to chelate calcium and magnesium and enhance trypsin activity. It is formulated on an HBSS base with the tetrasodium EDTA salt and phenol red indicator. After detachment, trypsin should be neutralised with serum-containing medium or a trypsin inhibitor.
How is Trypsin 0.25 % / 1 mM EDTA 4 Na in HBSS, w/o: Ca and Mg, with Phenol red used?
Aspirate medium, rinse the monolayer with Ca/Mg-free salt solution, add enough pre-warmed trypsin to cover the cells and incubate until they round up and detach; then neutralise, centrifuge and resuspend in fresh medium.
How is Trypsin 0.25 % / 1 mM EDTA 4 Na in HBSS, w/o: Ca and Mg, with Phenol red stored?
Store the solution at -20 °C. Stable 2 years from production.
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