Trypsin 0.25 % / EDTA 0.02 % in DPBS, w/o Ca & Mg
Trypsin 0.25 % / EDTA 0.02 % in DPBS, w/o Ca & Mg – Trypsin Cell Detachment Solution
Trypsin 0.25 % / EDTA 0.02 % in DPBS, w/o Ca & Mg, ready-to-use trypsin cell detachment solution
RUO (P10-020100)
Trypsin 0.25 % / EDTA 0.02 % in DPBS, w/o Ca & Mg is a ready-to-use cell detachment solution containing 0.25 % trypsin and 0.02 % EDTA, formulated in DPBS without calcium and magnesium. Trypsin is a porcine pancreas-derived enzyme (1:250) that dissociates anchorage-dependent cells; EDTA is added to chelate calcium and magnesium and enhance trypsin activity. This variant is supplied without phenol red for applications where the indicator dye could interfere. After detachment, trypsin should be neutralised with serum-containing medium or a trypsin inhibitor.
Reliable trypsin detachment for routine passaging. 0.25 % trypsin with 0.02 % EDTA in DPBS, Ca/Mg-free for effective dissociation.
Why Trypsin 0.25 % / EDTA 0.02 % in DPBS, w/o Ca & Mg
- Effective dissociation – porcine trypsin (1:250) for anchorage-dependent cells.
- EDTA-enhanced – EDTA chelates Ca/Mg to boost trypsin activity.
- DPBS base, Ca/Mg-free – formulated to support efficient trypsinisation.
- Neutralise after use – stop with serum-containing medium or trypsin inhibitor.
Formulation at a glance
| Type | Cell detachment solution |
| Trypsin | 0.25 % (1:250, porcine) |
| EDTA | 0.02 % EDTA |
| Base | DPBS, w/o Ca and Mg |
| Storage | -20 °C |
Ordering & grades
| Grade | Article No. | Size | Use | To Shop |
|---|---|---|---|---|
| RUO | P10-020100 | 100 ml | Research use | |
| RUO | P10-020500 | 500 ml | Research use |
Applications
- Routine detachment and passaging of adherent cells
- Subculture of anchorage-dependent cell lines
Frequently asked questions
What is Trypsin 0.25 % / EDTA 0.02 % in DPBS, w/o Ca & Mg?
Trypsin 0.25 % / EDTA 0.02 % in DPBS, w/o Ca & Mg is a ready-to-use cell detachment solution containing 0.25 % trypsin and 0.02 % EDTA, formulated in DPBS without calcium and magnesium. Trypsin is a porcine pancreas-derived enzyme (1:250) that dissociates anchorage-dependent cells; EDTA is added to chelate calcium and magnesium and enhance trypsin activity. This variant is supplied without phenol red for applications where the indicator dye could interfere. After detachment, trypsin should be neutralised with serum-containing medium or a trypsin inhibitor.
How is Trypsin 0.25 % / EDTA 0.02 % in DPBS, w/o Ca & Mg used?
Aspirate medium, rinse the monolayer with Ca/Mg-free salt solution, add enough pre-warmed trypsin to cover the cells and incubate until they round up and detach; then neutralise, centrifuge and resuspend in fresh medium.
How is Trypsin 0.25 % / EDTA 0.02 % in DPBS, w/o Ca & Mg stored?
Store the solution at -20 °C. Stable 2 years from production.
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