HBSS, w: Ca and Mg, w: 0.35 g/L NaHCO3
HBSS w: Ca and Mg, w: 0.35 g/L NaHCO3 – Hanks’ Balanced Salt Solution
HBSS, w: Ca and Mg, w: 0.35 g/L NaHCO3 – Hanks’ Balanced Salt Solution with phenol red
RUO (P04-32500) · GMP grade on request
Hanks’ Balanced Salt Solution (HBSS) with calcium and magnesium and 0.35 g/L sodium bicarbonate is a balanced salt solution that maintains pH and osmolality and provides essential inorganic ions. HBSS is designed for use with cells kept under non-CO2 atmospheric conditions. This variant contains the divalent cations Ca and Mg and phenol red as a pH indicator, and is used to wash and handle cells and as a carrier solution.
Hanks’ salts for non-CO2 handling. A balanced salt solution with Ca, Mg and phenol red for washing and handling cells.
Why HBSS, w: Ca and Mg, w: 0.35 g/L NaHCO3
- For non-CO2 conditions – HBSS is designed for atmospheric (non-CO2) handling.
- Contains Ca and Mg – divalent cations for adhesion-dependent steps.
- Phenol red – built-in pH indicator.
- Quality controlled – sterile and released to specifications.
Formulation at a glance
| Type | Balanced salt solution |
| Product | HBSS (Hanks’) |
| Ions | With Ca and Mg, w: 0.35 g/L NaHCO3 |
| Indicator | Phenol red |
| Storage | +2 °C to room temperature |
Ordering & grades
Applications
- Washing and handling under non-CO2 conditions
- Carrier and osmotic balance
Frequently asked questions
What is HBSS, w: Ca and Mg, w: 0.35 g/L NaHCO3?
Hanks’ Balanced Salt Solution (HBSS) with calcium and magnesium and 0.35 g/L sodium bicarbonate is a balanced salt solution that maintains pH and osmolality and provides essential inorganic ions. HBSS is designed for use with cells kept under non-CO2 atmospheric conditions. This variant contains the divalent cations Ca and Mg and phenol red as a pH indicator, and is used to wash and handle cells and as a carrier solution.
How is HBSS, w: Ca and Mg, w: 0.35 g/L NaHCO3 used?
Use as supplied for washing and handling cells maintained under non-CO2 conditions.
How is HBSS, w: Ca and Mg, w: 0.35 g/L NaHCO3 stored?
Store at +2 °C to room temperature. Stable 3 years from production.
Is a GMP-grade version available?
GMP grade is available on request for PAN-Biotech catalogue products (excluding sera), manufactured GMP EU Annex 1-compliant by PAN-Celltech.
One partner, from research through further manufacturing to GMP. Explore the full GMP-grade catalogue or use the Smart Product Search to find a matched product for your cell line.
Related: HBSS, w/o: Phenol red, w: Ca and Mg, w: 0.35 g/L NaHCO3 · HBSS, w/o: Ca and Mg, w: 0.35 g/L NaHCO3, w: Phenol red · GMP-Grade Products


