MEM Eagle with EBSS
MEM Eagle with EBSS – Classic Cell Culture Medium in Multiple Configurations
MEM Eagle with EBSS, classic cell culture medium
RUO (P04-08050) · GMP grade available (CT-08050)
MEM is an advancement of the BME and the base medium of many further modifications. Because BME did not fulfil all requirements for some mammalian and HeLa cells, a better variation had to be developed. Today, MEM is one of the most used synthetic media and shows its versatility by supplementing with amino acids including Hank`s or Earle`s salts. Even the addition of only small amounts of FBS results in a positive effect on cell growth. It is available in several configurations differing in glutamine form, so you can match the formulation to your protocol.
MEM Eagle with EBSS – available RUO and GMP. A matched GMP grade (CT-08050) is available for regulated and manufacturing workflows; choose the configuration that fits your cells.
Why MEM Eagle with EBSS
- Classic formulation – the established MEM Eagle with EBSS recipe for reliable, reproducible culture.
- Glutamine options – available as with L-glutamine, with stable glutamine, glutamine-free, with 2 mM L-glutamine.
- GMP available – matched GMP grade CT-08050 for manufacturing.
- Multiple pack sizes – available in several pack sizes; other sizes on request.
Formulation at a glance
| Type | Classic basal cell culture medium |
| Product | MEM Eagle with EBSS |
| Configurations | glutamine form |
| GMP grade | CT-08050 (on request) |
| Format | Liquid, sterile-filtered |
| Storage | +2°C – +8°C |
Ordering & grades
| Grade | Article No. | L-Glutamine | Size | Use | To Shop |
|---|---|---|---|---|---|
| RUO | P04-08050 | w/o | 500 ml | Research use | |
| GMP | CT-08050 | 500 ml | GMP-compliant, on request | ||
| RUO | P04-085001 | with | 1000 ml | Research use | |
| P04-08500 | 500 ml | ||||
| GMP | CT-08500 | 500 ml | GMP-compliant, on request | ||
| RUO | P04-09500 | stable | 500 ml | Research use | |
| RUO | P04-08056 | 2 mM | 500 ml | Research use | |
| GMP | CT-08056 | 500 ml | GMP-compliant, on request |
Applications
- General mammalian cell culture
- Routine culture with serum supplementation
- GMP manufacturing workflows
Frequently asked questions
What is MEM Eagle with EBSS?
MEM (Minimum Essential Medium) is an advancement of BME and the base medium for many further modifications. Today it is one of the most widely used synthetic media; it works with Hanks’ or Earle’s salts, and even small amounts of FBS have a positive effect on cell growth.
Which configurations are available?
Options include with L-glutamine, with stable glutamine, glutamine-free, with 2 mM L-glutamine.
Is a GMP grade available?
Yes. A GMP grade (CT-08050) is available on request for regulated and manufacturing use.
How is it stored?
MEM Eagle with EBSS is stored at +2°C – +8°C.
One partner, from research through further manufacturing to GMP. Explore the full GMP-grade catalogue or use the Smart Product Search to find a matched product for your cell line.
Related: DMEM · RPMI 1640 · GMP-Grade Products


