Ham’s F12 Medium
Ham’s F12 Medium – Classic Cell Culture Medium in Multiple Configurations
Ham’s F12 Medium, classic cell culture medium
RUO (P04-14550)
Ham’s F12 was developed for the serum-free clonal growth of Chinese Hamster Ovary (CHO) cells, lung cells and mouse L-cells. It can be used for serum free growth of CHO cultures supplemented with hormones, selenium and other supplements as well as for other mammalian cells especially rodent and other mammalian cell lines (particularly rat and rabbit). It is available in several configurations differing in glutamine form, so you can match the formulation to your protocol.
Classic Ham’s F12 Medium for reliable cell culture. A well-established formulation, sterile-filtered and quality-controlled for reproducible results.
Why Ham’s F12 Medium
- Classic formulation – the established Ham’s F12 Medium recipe for reliable, reproducible culture.
- Glutamine options – available as with L-glutamine, glutamine-free.
- Broad use – suitable for a wide range of routine cell culture applications.
- Multiple pack sizes – available in several pack sizes; other sizes on request.
Formulation at a glance
| Type | Classic basal cell culture medium |
| Product | Ham’s F12 Medium |
| Buffer | 1.176 g/L NaHCO3 |
| Configurations | glutamine form |
| Format | Liquid, sterile-filtered |
| Storage | +2°C – +8°C |
Ordering & grades
Applications
- General mammalian cell culture
- Routine culture with serum supplementation
Frequently asked questions
What is Ham's F12 Medium?
Ham’s F12 was developed for the serum-free clonal growth of Chinese Hamster Ovary (CHO) cells, lung cells and mouse L-cells. It can be used for serum-free CHO culture supplemented with hormones and selenium, and for other mammalian cells, especially rodent cell lines.
Which configurations are available?
Options include with L-glutamine, glutamine-free.
How is it stored?
Ham’s F12 Medium is stored at +2°C – +8°C.
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