Accutase Cell Detachment Solution, w: 0.5 mM EDTA, w: Phenol red
Cell dissociation reagent · RUO (P10-21500)
Accutase is a gentle, non-mammalian, non-bacterial cell-detachment solution (with 0.5 mM EDTA and phenol red) that dissociates adherent cells without the harshness of trypsin, preserving surface epitopes and viability.
One catalogue quality. Accutase Cell Detachment Solution, w: 0.5 mM EDTA, w: Phenol red is a RUO product (P10-21500), manufactured by PAN-Celltech and released against a defined quality-control panel on every lot.
Why
- Gentle dissociation – non-mammalian enzyme; kinder to surface markers and viability than trypsin.
- Ready to use – no neutralisation step required.
- Defined – with 0.5 mM EDTA and phenol red indicator.
Specification
| Composition | Non-mammalian dissociation enzyme with 0.5 mM EDTA, phenol red |
| Form | Ready-to-use solution |
| Grade | RUO |
| Storage | -20 °C (in the dark); thawed material at 2-8 °C for up to 1 month |
Ordering
| Grade | Art. No. | Pack size |
|---|---|---|
| RUO | P10-21500 | 500 ml |
Other pack sizes on request.
In the process – by application (upstream & downstream)
Accutase Cell Detachment Solution, w: 0.5 mM EDTA, w: Phenol red is used at the following process steps across the biologics platforms it supports:
Monoclonal antibodies
- Screening & Selection (Hybridoma / Seed Train) – Gentle cell dissociation for adherent hybridoma clones; subculture.
- Harvest & Clarification – Gentle adherent cell harvest without trypsin.
Cell & gene therapy
- Dissociation – Cell dissociation / passaging.
Vaccines
- Cell dissociation – Gentle cell dissociation.
Frequently asked questions
In which grade is Accutase Cell Detachment Solution, w: 0.5 mM EDTA, w: Phenol red available?
It is available as a RUO product (P10-21500); a GMP grade is not available.
Which processes is this product used in?
Accutase Cell Detachment Solution, w: 0.5 mM EDTA, w: Phenol red is used across the PAN Cell & gene therapy and Vaccine production processes. See "In the process – by application (upstream & downstream)" above for the specific steps in each.
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