Sterile Water for Cell Culture
Ultrapure Water for Research
Ultrapure, sterile-filtered (0.2 µm) water for research and routine cell-culture workflows · RUO · non-GMP
Sterile Water for Cell Culture is an ultrapure, sterile-filtered (0.2 µm) research-grade water for cell-culture and laboratory use. Sterile Water for Cell Culture is an ultrapure, ready-to-use water produced by reverse osmosis, ultrafiltration and electrodeionization, then sterile-filtered to 0.2 µm. Every batch is quality-controlled, including sterility testing and verification of key physicochemical parameters.
One partner, across the whole purity ladder. PAN-Biotech offers three matched water grades — Sterile Water for Cell Culture (research), WFI Quality Water (GMP, EP/USP) and WFI Beyond (beyond compendial, FDA DMF) — so the same partner carries you from research to GMP without changing supplier.
Why Sterile Water
Ultrapure & sterile
Sterile-filtered to 0.2 µm, with a contamination-free aqueous base.
Ready to use
No further treatment needed for routine bench work.
Low endotoxin
Consistent quality where full pharmacopoeial WFI is not mandatory.
Batch-controlled
Comprehensive QC including sterility testing.
Flexible formats
From small bottles to a 20 L bag.
Research economy
The right grade for non-GMP laboratory workflows.
Specifications at a glance
| Property | Detail |
|---|---|
| Water grade | Ultrapure, sterile (non-GMP) |
| Sterile filtration | 0.2 µm |
| Production | Reverse osmosis · ultrafiltration · electrodeionization |
| Endotoxin | Low |
| QC | Sterility testing + key physicochemical parameters |
For GMP or pharmacopoeial water, see WFI Quality Water (EP/USP) or WFI Beyond (beyond compendial).
Formats and article numbers
Sterile Water for Cell Culture is the research-grade (RUO) water for the bench and early development - with a direct, no-reformulation path to the identical water in GMP. Bottles and single-use bags; larger and custom formats on request.
Sterile Water for Cell Culture - research (RUO)P04-99 series
- 500 ml bottleP04-991500
- 1000 ml bottleP04-991000
- 20 L bagP04-99200B
One water, from bench to GMP
The research grade (P-series) and the GMP grades (CT-series) run on a matched continuum: develop in research-grade sterile water and move to the identical qualified GMP water - WFI Quality Water to the EP/USP monograph, or WFI Beyond beyond it - with no reformulation. Qualify once, scale without re-qualifying.
Research use (RUO). Animal- and antibiotic-free, sterile-filtered, made in Germany. For GMP media, buffer and formulation preparation, see WFI Quality Water (CT-series).
Applications
Key terms
| Term | Meaning |
|---|---|
| WFI | Water for Injection — the pharmacopoeial grade of water permitted in injectable medicines (Ph. Eur. monograph 0169). |
| Endotoxin | Pyrogenic fragments of bacterial cell walls; the Ph. Eur. WFI limit is < 0.25 EU/mL. |
| TOC | Total organic carbon — a measure of organic purity; the WFI monograph limit is 0.5 mg/L. |
| Nuclease-free | No detectable RNase or DNase activity — protects RNA/DNA and mRNA payloads. |
| ICH Q3D | The international guideline that sets safe limits for 24 elemental (metal) impurities in medicines. |
| SAL 10⁻⁶ | Sterility Assurance Level of one-in-a-million — the pharmaceutical standard for terminal sterilisation. |
| DMF | Drug Master File — confidential data filed with the FDA that a customer references in their own submission via a letter of authorization. |
| GMP (EU Annex 1) | Good Manufacturing Practice for the manufacture of sterile medicinal products in the EU. |
Frequently asked questions
Is Sterile Water for Cell Culture a GMP product?
No — it is a research-grade (non-GMP) ultrapure water for routine laboratory and cell-culture use. For GMP-regulated work, see WFI Quality Water; for beyond-compendial performance, see WFI Beyond.
Is it sterile?
Yes. It is sterile-filtered to 0.2 µm and each batch undergoes sterility testing.
What is it used for?
Media preparation, reagent dilution, buffer formulation and analytical procedures where low endotoxin and consistent quality are needed but full pharmacopoeial WFI compliance is not required.
What sizes are available?
500 ml and 1 L bottles and a 20 L bag (P04-991500 / P04-991000 / P04-99200B).