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Upstream & Downstream

Tris Buffer 1 M, pH 8.4

1 M Tris-HCl buffer concentrate at pH 8.4 for equilibration and neutralisation in downstream processing. · GMP catalogue product · CT-05500

GMP · CT-05500Downstream · DSP

Tris Buffer 1 M, pH 8.4 is a defined buffer concentrate for downstream processing. It is diluted to working strength for column equilibration and used for neutralisation / pH re-adjustment, for example after a low-pH viral-inactivation hold. As a concentrated, GMP-grade base it is used across monoclonal antibodies, vaccines, mRNA and cell & gene therapy.

One GMP quality. Tris Buffer 1 M, pH 8.4 is a GMP-grade catalogue product (CT-05500), manufactured by PAN-Celltech and released against a defined quality-control panel on every lot.

Why

  • Concentrated 1 M base - Dilute to the required working strength.
  • Reliable neutralisation - Defined pH 8.4 ± 0.2 for post-inactivation adjustment.
  • GMP grade - Catalogue GMP quality, released per lot.
  • Multi-application - One base across antibody, vaccine, mRNA and C&G downstream.

Specification

Composition1 M Tris-HCl
pH8.4 ± 0.2
FormBuffer concentrate
Storage15-25 °C (room temperature)
GradeGMP

Ordering

GradeArticle No.Pack size
GMPCT-05500500 ml

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Other pack sizes are available on request.

Beyond compendial - on request. Every PAN downstream buffer is also available in a beyond-compendial version produced with WFI Beyond - Water for Injection engineered beyond the Ph. Eur./USP monograph - for the most demanding endotoxin and purity requirements. Available on request.

In the downstream process - by application

Tris Buffer 1 M, pH 8.4 is used at the following process steps across the four biologics platforms:

Monoclonal antibodies

  • Protein A capture - equilibration buffer base.
  • Viral inactivation - neutralisation / pH re-adjustment after the low-pH hold.
  • Ion exchange - equilibration and pH adjustment.

Vaccines

  • Chromatography - equilibration buffer base.
  • Post-treatment - neutralisation and pH adjustment.

mRNA

  • Handling / storage - TE-type buffering (Tris base).
  • Buffer exchange / formulation - buffering to target pH.
  • Reactions - neutralisation / reaction stop.

Cell & gene therapy

  • Chromatography - equilibration and neutralisation.
  • Lysis / handling - TE-buffer component.
  • Plasmid DNA - process buffering.

Frequently asked questions

What is Tris Buffer 1 M, pH 8.4 used for?

It is a concentrate for column equilibration and for neutralisation / pH re-adjustment after low-pH steps.

What is the pH?

The concentrate is supplied at a fixed pH of 8.4 ± 0.2.

Is it multi-application?

Yes. It is used across monoclonal antibodies, vaccines, mRNA and cell & gene therapy downstream.

One partner across every application. The same GMP downstream buffers support monoclonal antibodies, vaccines, mRNA and cell & gene therapy - explore the full GMP-grade catalogue or use the Smart Product Search.

Related: WFI Quality Water (CT-991500) · DPBS w/o Ca & Mg (CT-36500) · GMP-Grade Products