Endonuclease Solution
Broad-range endonuclease for host-cell and residual DNA/RNA digestion — nucleic-acid clearance and viscosity reduction in clarification, lysate treatment and polishing. · GMP catalogue product · CT-77351
Endonuclease Solution is a broad-range nuclease for downstream processing. It digests host-cell and residual DNA/RNA to reduce viscosity and clear nucleic acids during clarification, lysate treatment and polishing — and removes IVT template in mRNA workflows.
Why
- Broad-range digestion — host-cell and residual DNA/RNA.
- Viscosity reduction — clears nucleic acids in clarification and lysate treatment.
- GMP grade — for regulated processing.
- Multi-application — mRNA, cell & gene therapy and vaccines.
Specification
| Composition | Broad-range endonuclease |
| Storage | -20 °C |
| Form | Enzyme solution (glycerol-based) |
| Grade | GMP |
Ordering
| Grade | Article No. | Pack size |
|---|---|---|
| GMP | CT-77351 | 100 ml |
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Other pack sizes are available on request.
In the downstream process — by application
Endonuclease Solution is used at the following process steps across the biologics platforms it supports:
mRNA
- IVT template removal — digestion of the DNA template after transcription.
Cell & gene therapy
- Lysate treatment — host-cell DNA/RNA clearance and viscosity reduction.
- Polishing — residual nucleic-acid clearance.
Vaccines
- Clarification — host-cell nucleic-acid reduction.
Frequently asked questions
What is Endonuclease Solution used for?
It is a broad-range nuclease for digesting host-cell and residual DNA/RNA — clearance and viscosity reduction in clarification, lysate treatment, polishing and IVT template removal.
How is it stored?
At -20 °C; the glycerol-based solution should not undergo repeated freeze/thaw.
Related: WFI Quality Water (CT-991500) · DPBS w/o Ca & Mg (CT-36500) · GMP-Grade Products