Glycine 100 mM Buffer, pH 3.0
Ready-to-use 100 mM glycine buffer at pH 3.0 for low-pH elution and inactivation steps in downstream processing. · GMP catalogue product · CT-69500
Glycine 100 mM Buffer, pH 3.0 is a defined, ready-to-use low-pH buffer. In downstream processing it serves mainly as a low-pH elution buffer for affinity (Protein A) capture - an alternative to citrate - and for low-pH hold steps. As a GMP-grade buffer with a fixed composition and pH it is used broadly across biologics platforms, from monoclonal antibodies, vaccines, mRNA and cell & gene therapy.
Why
- Defined low pH - Fixed pH 3.0 ± 0.2 for reproducible elution.
- Alternative to citrate - For Protein A platforms that prefer a glycine system.
- GMP grade - Catalogue GMP quality, released per lot.
- Multi-application - One buffer across antibody, vaccine, mRNA and C&G downstream.
Specification
| Composition | 100 mM glycine |
| pH | 3.0 ± 0.2 |
| Form | Ready-to-use low-pH buffer |
| Storage | 15-25 °C (room temperature) |
| Grade | GMP |
Ordering
| Grade | Article No. | Pack size |
|---|---|---|
| GMP | CT-69500 | 500 ml |
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Other pack sizes are available on request.
In the downstream process - by application
Glycine 100 mM Buffer, pH 3.0 is used at the following process steps across the four biologics platforms:
Monoclonal antibodies
- Protein A capture - alternative low-pH elution.
- Viral inactivation - low-pH hold where a glycine system is preferred.
Vaccines
- Affinity / IEX - acidic elution or low-pH hold where used.
mRNA
- Not typically used in this modality.
Cell & gene therapy
- Affinity capture (AAV / viral vectors) - low-pH elution.
Frequently asked questions
What is Glycine 100 mM Buffer used for?
It is used mainly as a low-pH elution buffer for Protein A affinity capture and for low-pH hold steps in downstream processing.
What is the pH?
It is supplied at a fixed pH of 3.0 ± 0.2.
Can it be used outside antibody processes?
Yes. It is a general low-pH buffer applicable across monoclonal antibodies, vaccines, mRNA and cell & gene therapy downstream, wherever a defined low-pH buffer is required.
Related: WFI Quality Water (CT-991500) · DPBS w/o Ca & Mg (CT-36500) · GMP-Grade Products