Frequently asked questions

Amniopan is tested for sterility by membrane filtration.

Yes, Amniopan contains gentamicin.

As a liquid medium, Waymouth’s MB 752/1 is available only with NaHCO₃ (P04-28500). Without NaHCO₃ it is available as a powder (P03-4510 and P03-4550).

2x MEM Eagle contains all components at double concentration. It should be diluted 1:1 with sterile water — for example, Water for Cell Culture.

If a required formulation is not part of our catalog, we can calculate and produce it as a custom medium. Please contact us with your exact specifications — for example, components to include or omit, buffering, and whether you need it as a liquid or powder.

The product has a concentration of 0.25 mg/ml.

For freezing media free of animal and human components, we recommend Cryopan II and Cryopan III. Cryopan I is serum-free but not free of animal components.

Overview: Cryopan I — serum-free (P07-92100); Cryopan II — additionally protein-free (P07-93100); Cryopan III — DMSO-free, not protein-free (P07-96100). All three are also part of our GMP standard portfolio (Cryopan I: CT-92100, Cryopan II: CT-94100, Cryopan III: CT-96100).

For initial testing, the RUO-grade standard variants can be used for research purposes. Please contact us regarding current GMP availability.

We recommend aliquoting the product. We do not have data on the shelf life of Cryopan at refrigerator temperatures.

The color change is caused by the phenol red pH indicator in the medium. The pH naturally shifts downward during culture due to cellular metabolites, and some cell types are sensitive to pH changes.

Because the effect of a given pH deviation depends on your specific cells and process, we cannot issue a general clearance. If a risk assessment on your side shows that a deviation in this range is not critical, the medium can be used; otherwise, we recommend replacing the bottle to be safe.

It contains heparin. The Panexin SL-S supplement also contains small amounts of animal-derived components, which cannot be specified further due to the proprietary composition.

Yes, gelatin coating should always be performed.

The growth curve in the Endopan 300 SL data sheet was generated with the HUVEC/TERT66 cell line. This line has a relatively slow doubling time and does not reach as high a confluence as some other cell lines.

Due to the proprietary composition of the kits, we do not disclose the exact concentrations of the individual supplements. Please refer to the product data sheet for handling and usage details.

UV light can have a negative effect on medium components and may affect cell growth. We do not have data quantifying the impact of a 30-minute exposure.

Cells being thawed (e.g. HUVECs) can be particularly sensitive at that stage. If you have more than one bottle, we recommend using a fresh one to be safe — or at least not thawing all cells with the affected medium at once, so you can first test whether it has any negative effect before committing the full batch.

For short transport durations, brief excursions to ambient temperature are generally not critical for this product. If you are unsure about the specific conditions your shipment experienced, please contact us with the details so we can advise.

No. The kit includes a lipid supplement and a supplement containing iron-loaded deferiprone, which can partially replace holotransferrin.

Our endotoxin test has a detection limit of 0.005 EU/ml. Because protein-rich substances such as FBS interfere with the endotoxin assay, undiluted samples tend to perform poorly, so FBS is tested in a dilution — for example 1:100.

When no endotoxins are detected in a 1:100 dilution, the result of < 0.005 EU/ml must be back-calculated to the undiluted sample by multiplying by the dilution factor (×100). This gives a final reported value of < 0.5 EU/ml, which represents a low-endotoxin serum.

We do not test our serum for methionine content.

Delipidization reduces the lipid content rather than removing it completely. We test delipidized FBS for cholesterol and triglycerides: cholesterol is markedly reduced, while triglycerides decrease only slightly. We do not separately quantify phospholipids or other lipid classes.

On request, the Certificates of Analysis (CoA) of a standard FBS lot and its delipidized counterpart can be compared to illustrate the effect of delipidization.

Such particles are usually fibrin. They do not affect the performance of the serum, but can be visually disturbing under the microscope — for example when counting cells.

The particles can be removed by filtration (e.g. 0.22 µm) or by centrifugation. After removal, the product can be used normally.

These particles are usually not bacteria — they are typically precipitates, such as calcium phosphate. They do not affect product performance and can be removed by centrifugation if they are visually disturbing.

If you want to rule out microbial contamination, a sample can be assessed microbiologically, for example by plating on TSB agar.

Truly EV-free serum is very difficult to provide, because EVs are a highly diverse fraction of serum. The most feasible approach would be depletion by ultrafiltration or dialysis, but this works purely on the basis of size and would also remove other components such as proteins and hormones — it is not specific to EVs.

We do not have a standardized routine for this type of serum modification. To assess feasibility, we would need more information about the nature and the size cut-off of the EVs in question.

Yes, HybridBoost contains 4.5 g/L glucose.

We offer ISF-1 (P04-995968), Panserin H8000 (P04-718000) and HybridBoost (P04-995910). HybridBoost is our own formulation developed for hybridoma culture.

We currently do not have data on the culture of High Five cells in Spodopan, nor a direct comparison of growth rate and protein expression between Sf9 and High Five cells in this medium.

No. Internally we use Spodopan in a standard incubator without additional CO₂, at a temperature of 28 °C.

Usually the samples are diluted 1:10 (1 part sample and 9 parts medium). Higher sample concentrations such as 1:5 should generally be possible, although we do not have specific data on this. Note that higher cell densities can cause nutrients to be consumed more quickly.

We recommend monitoring the color change of the pH indicator and performing a medium change if needed, so that sufficient nutrients are available throughout the culture.

Neuropan has primarily been tested with rat neurons. We currently do not have internal data or published references on its performance with mouse neurons or iPSC-derived NGN2-induced neurons.

Pancoll is optimized for the isolation of cells from (whole) blood, and we do not have data for isolation from other tissues. To select the correct Pancoll, the densities of both the target cells (lymphocytes) and the cells to be separated from them (e.g. epithelial cells) need to be known.

Layering two different Pancoll variants is possible — this is done, for example, for granulocytes, for which a separate instruction sheet is available.

We do not add IL-2Rα to Panexin NTA, and the product is not tested for the presence of this component. It is unlikely to be introduced as a contaminant from other raw materials.

If you have detected it — for example by Western blot — we recommend confirming that the signal originates from Panexin NTA itself and not from other elements of your system, such as culture media, reagents or cell-culture supernatants.

Panexin CD and Panexin NTA should both be suitable. For macrophage cell lines we additionally recommend Panexin BMM, which is designed specifically for macrophages.

Panexin CD does contain hormones. However, due to the proprietary composition we cannot specify which hormones are present or their concentrations.

Options to support specific projects may include a confidentiality agreement, or the production of a hormone-free variant of Panexin CD on request.

We do not offer an HSA with these specifications as a standard product. A custom project may be possible — please contact us to discuss your requirements and quantities.

We do not offer a direct equivalent to TrypLE. Since TrypLE is a trypsin alternative, we can offer Accutase, which is likewise a trypsin alternative for cell detachment.

Our alternative is P04-08056 (EMEM). Note that some components may differ slightly in g/L concentration from the ATCC formulation.

We recommend the PANcell CHO Feed Kit.