Vaccine Production
The development and production of vaccines is important to prevent and treat diseases. While traditional vaccines are often produced with the use of chicken eggs, cell culture based approaches are another viable method to manufacture vaccines.
The manufacture of cell culture based vaccines consists of several important steps. Suitable established cell lines have to be selected or developed. These cell lines, e.g. MDCK, HEK293 or Vero, are transfected with specific viruses, which replicate within the cells. The viruses are then harvested and purified, before the vaccine is formulated for administration and storage.
Our products are used during these steps of cell culture based vaccine production. We provide vaccine manufacturers with cell culture media, supplements and freezing media for cell line development and cell expansion, customized media for virus transfection as well as buffer and enzyme solutions for viral harvesting, purification and vaccine formulation.
Upstream (USP)
Downstream (DSP)
Cell Line Development and
Maintenance
Virus Infection / Transfection
Cell expansion
Viral Harvesting and
Purification
Vaccine Formulation
(Excipients Grade)
- Culture Media: e.g. DMEM/F12, RPMI 1640
- Antibiotics: e.g. Penicillin-Streptomycin
- Growth Factors: e.g. EGF, FGF
- Trypsin-EDTA solution (for cell detachment)
- Buffered Saline Solutions: e.g. PBS
- Cryopreservation media:
- Cryopan
- Customized Media
- WFI Quality Water
- Transfection Reagents: e.g. Lipofectamine, FuGENE)
- Viral Vector Systems: e.g. Lentivirus, Adenovirus vectors
- siRNA or shRNA (for gene silencing, if applicable)
- Polybrene (to enhance viral transduction efficiency)
- Customized Serum-Free Transfection Media
- Customized Buffer Solutions
- WFI Quality Water
- Customized Cell Culture Media
- Culture Media: e.g. DMEM/F12, RPMI 1640
- Cell dissociation buffers
- Cryopreservation media: Cryopan
- Balanced Salt Solutions: e.g.
Hanks’ or Earle’s - Cell Culture Supplements: e.g.
Insulin, Transferrin - WFI Quality Water
- Cell Lysis Reagents: e.g. Triton X-100, Sodium Dodecyl Sulfate (SDS), Nonidet P-40 (NP-40), RIPA Buffer, Lysozyme
- Nuclease Solutions: e.g. Benzonase Nuclease
- Proteinase Inhibitors (EDTA)
- Customized Concentration Buffers
- WFI Quality Water
- Adjuvants
- Stabilizers
- Preservatives
- Customized Buffer Solutions: e.g. Tris, Sodium Acetate
- Customized Diluents: e.g. 0.9% Sodium Chloride, Ringer’s Solution, Balanced Salt Solution
- WFI Quality Water
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