Antibody Production
Monoclonal antibodies represent a groundbreaking advancement in the field of medicine. They detect and bind to specific epitopes, the parts of an antigen which are recognized by the immune system. Polyclonal antibodies, which are derived from multiple cell lines, recognize various epitopes, whereas monoclonal antibodies are produced from a single cell line and target one specific epitope. This specificity has paved the way for targeted therapies in various diseases.
Antibody producing cells can be created by using the hybridoma technology. This technique involves fusing B cells with myeloma cells. The resulting hybridoma cells have the antibody-producing ability of the B cells and the longevity and robustness of myeloma cells.
We manufacture media, supplements and reagents which are essential for the upstream and downstream phase of antibody production. With our off-the-shelf products as well as customized formulations we offer suitable solutions for the generation of hybridoma cells and screening as well as antibody production and purification.
Upstream (USP)
Downstream (DSP)
Immunization and
Hybridoma Generation
Screening and Selection
Large-Scale Antibody
Production
Harvest and
Clarification
Polishing and Formulation
(Excipients Grade)
- Adjuvants: e.g. Freunds’ Complete and Incomplete Adjuvant
- Antigens (specific to the target of the antibody)
- Customized Cell Fusion Reagents: e.g. Polyethylene Glycol for hybridoma production
- Hybridoma Growth Supplements
- WFI Quality Water
- ELISA Kits (for screening antibody specificity and affinity)
- Cell Culture Media: e.g. DMEM, RPMl-1640
- Antibiotics: e.g. Penicillin-Streptomycin
- WFI Quality Water
- Bioreactor Culture Media (specifically formulated for the
chosen cell line) - Feed Supplements (to enhance cell growth and productivity)
- Cell Culture Additives: e.g. anticlumping agents
- Customized Cell
Lysis Reagents (if
intracellular
antibodies) - Buffer Solutions: e.g.
PBS for wash steps
- Customized Buffer Solutions for Chromatography
- Concentration Buffers: e.g. for ultrafiltration
- Stabilizers: e.g. Sucrose, Trehalose
- Preservatives (if required for storage)
Products highlighted in orange are currently available.